HTB

时间 : 2009-11-29 08:01:40 来源：www.chem17.com

[摘要]

恒大百盛生物（北京）科技发展有限公司上海办事处专业供应销售HTB-138Hs 683（人脑视神经胶质瘤细胞），欢迎您来电咨询HTB-138Hs 683（人脑视神经胶质瘤细胞）的详细信息！恒大百盛生物（北京）科技发展有限公司上海

【详细说明】

Hs683（人脑视神经胶质瘤细胞）CellBiologyATCC?Number:HTB-138?Price:$255.00Designations:Hs683Depositors:RBOwensBiosafetyLevel:1Shipped:frozenMedium&Serum:SeePropagationGrowthProperties:adherentOrganism:Homosapiens（human）Morphology:fibroblastSource:Organ:brainDisease:gliomaPermits/Forms:InadditiontotheMTAmentionedabove,otherATCCand/orregulatorypermitsmayberequiredforthetransferofthisATCCmaterial.AnyonepurchasingATCCmaterialisultimatelyresponsibleforobtainingthepermits.Pleaseclickhereforinformationregardingthespecificrequirementsforshipmenttoyourlocation.RelatedCellCultureProductsTumorigenic:No,inimmunosuppressedmiceYes,insemisolidmediumDNAProfile（STR）:Amelogenin:X,YCSF1PO:9,13D13S317:8,12D16S539:9,10D5S818:11,12D7S820:11THO1:6,8TPOX:8,11vWA:18,20CytogeneticAnalysis:modalnumber=82;range=74to87.Thisisahypertriploidhumancelllinewiththemodalchromosomenumberof82occurringin16%ofcells,withthedistributionskewedtowardthelowernumberrange.Higherploidiesoccurredin3.0%ofcells.Mostcellshad4-6markerchromosomes,butonlydel（3）（q12.2q21）occurredfrequentlyinmostcells,ａndder（9）t（9;?）（q34.2;?）,tdic（1;4）（q21;q35）,ａndaminuteacrocentricoccurredinmorethantwocells.N7occurredin4-6copiespercell.TwoXａndoneortwoYchromosomeswerepresentinmostcells.Isoenzymes:AK-1,1;ES-D,1;G6PD,B;GLO-I,2;Me-2,2;PGM1,1;PGM3,1-2Age:76yearsGender:maleEthnicity:CaucasianComments:Hs683wasisolatedfromexplantculturesofagliomatakenfromthelefttemporallobeofa76yearoldmaleCaucasian.Microvillibutnodesmosomeswereobserved.Propagation:ATCCcompletegrowthmedium:ThebasemediumforthiscelllineisATCC-formulatedDulbecco＇sModifiedEagle＇sMedium,CatalogNo.30-2002.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%.Temperature:37.0CAtmosphere:air,95%;carbondioxide（CO2）,5%Subculturing:Protocol:Removeａnddiscardculturemedium.Brieflyrinsethecelllayerwith0.25%（w/v）Trypsin-0.53mMEDTAsolutiontoremovealltracesofserumthatcontainstrypsininhibitor.Add2.0to3.0mlofTrypsin-EDTAsolutiontoflaskａndobservecellsunderaninvertedmicroscopeuntilcelllayerisdispersed（usuallywithin5to15minutes）.Note:Toavoidclumpingdonotagitatethecellsbyhittingorshakingtheflaskwhilewaitingforthecellstodetach.Cellsthataredifficulttodetachmaybeplacedat37°Ctofacilitatedispersal.Add6.0to8.0mlofcompletegrowthmediumａndaspiratecellsbygentlypipetting.Addappropriatealiquotsofthecellsuspensiontonewculturevessels.Incubateculturesat37°C.

Subcultivationratio:Asubcultivationratioof1:4isrecommended

Mediumrenewal:TwiceperweekPreservation:Freezemedium:Completegrowthmediumsupplementedwith5%（v/v）DMSOStoragetemperature:liquidnitrogenvaporphaseReferences:22534:OwensRB,etal.Epithelialcellculturesfromnormalａndcanceroushumantissues.J.Natl.CancerInst.56:843-849,1976.PubMed:17641223094:OlopadeOI,etal.Molecularanalysisofdeletionsoftheshortarmofchromosome9inhumangliomas.CancerRes.52:2523-2529,1992.PubMed:156822123221:GershwinME,etal.Immunobiologyofheterotransplantedhumantumorsinnudemice.J.Natl.CancerInst.58:1455-1463,1977.PubMed:85703332550:DebinskiW,etal.Receptorforinterleukin（IL）13doesnotinteractwithIL4butreceptorforIL4interactswithIL13onhumangliomacells.J.Biol.Chem.271:22428-22433,1996.PubMed:8798406

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