平阳霉素局部注射治疗瘢痕疙瘩的实验研究

【摘要】目

【关键词】平阳霉素;瘢痕疙瘩;Ⅲ型胶原

　　AbstractObjective:tofurtherexploretherolemechanismofPingyangmycintreatmentionkeloids.Methods:Specimensfrom126casesofKeloidfromtheDepartmentofPathologyinRenminHospitalofWuhanUniversitywerecollected.126caseKeloidpatientswererandomlydividedintotwogroups:Treatmentgroup（groupA：n=74）andcontrolgroup（GroupB:n=52）.Treatmentgroup:Pingyangmycinfrom8mg,2%lidocaine5mldiluted,sincetheneedlescaredge,tipofthecentralscar,sinceneedleinjectionsitewerethefan-likeuniformtoshowscarsorpaleappearanceforthekindoforangepeel.Controlgroup:prednisoloneacetateinjection125mgdissolvedin2%lidocainehydrochlorideinjection,5ml,lesionstreatedatthebaseclosed.Injectionandtreatmentmethodsweresamewiththetreatmentgroup.ThespecimensweregetedSixmonthsforeachgroupbasedtreatment,andthecontentofⅢcollagenfiberwereobservedbyhistologicalandhistologicalchemistry.ThepositiveareaofⅢcollagenfiberwereanalyzedusingimageanalysisImageHPIAS-2000AnalysisSystem.Results:ⅢcollagenfiberswereintensivedistributioninAgroup,ⅢcollagenfiberswerethesparsedistributioninBGroup.Imageanalysiswasshowedthat:ThepositiveareaofⅢcollagenfibreswerebetweenAGroupandBGrouphadstatisticallysignificantdiscrepancy（P<0.01）.Conclusion:PingyangmycincanmakescartissuefibroblastsDNAbreakage,collagenousfibersformatingtodestroy,andpromotecollagendissolved

KeywordsPYM;keloid;Ⅲcollagen

瘢痕疙瘩

平阳霉素为一种抗肿瘤药物

　　1材料与方法

　　1.1材料

　　1.1.1主要药品及器械

2%利多卡因

　　1.1.2患者来源

　　参加治疗

　　1.2方法

　　1.2.1临床治疗

　　A组：常规行血常规和胸部透视

B组：用醋酸泼尼松龙注射液125mg溶于2%盐酸利多卡因注射剂5ml

　　1.2.2组织学切片

所有瘢痕疙瘩标本均源于我院自愿行瘢痕疙瘩注射治疗后

　　1.2.3切片制作

常规HE染色：取材、固定、脱水、透明、包埋、切片、HE染色及封片

组织化学染色（苦味酸-天狼猩红染色）：①脱蜡

　　1.3结果判断

出现绿色染色

采用HPIAS-2000高清晰度彩色病理图文报告管理系统（同济千屏影像公司）对苦味酸-天狼猩红染色进行定量分析

　　1.4统计学处理

数据以均数±标准差（x±s）表示

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　　2.1HE染色

光镜下可见A组

　　2.2组织化学染色

A组在光镜下可见瘢痕组织中以散在分布