B01115人胶质母细胞瘤细胞

骨骼是一类动态的组织，被破骨细胞系统和造骨细胞系统的调节活动不断重塑。造骨细胞是骨骼形成细胞，最初是来源于多能性的间叶干细胞。它们分泌、合成细胞外基质和主要含有I型胶原的的类骨质。类骨质被造骨细胞钙化，在此过程中，细胞在钙化物质中嵌入陷窝中变成骨细胞。当骨骼发育成熟时，造骨细胞的数量和大小减少，但休眠细胞依然可以对伤害做出反应，在骨折愈合过程中产生新的骨骼。在生命的后期，造骨细胞的活动受到甲状旁腺素循环水平的直接影响。造骨细胞表达蛋白酶活性受体1和血管内皮细胞生长因子。研究表明，无论在体内或体外，白血病抑制因子都能与造骨细胞表面结合诱导骨骼形成。颅骨缝合处造

Boneisadynamictissue,beingcontinuouslyremodeledbythecoordinatedactionsofosteoclastsａndosteoblastlineage.Osteoblasts,thebone-formingcells,arederivedoriginallyfrompluripotentmesenchymalstemcells.Theysynthesizeａndsecreteanorganicextracellularmatrix,osteoid,whichiscomposedprimarilyoftypeIcollagen.Osteoidiscalcifiedbyosteoblastsａndduringthisprocessthecellsbecomeencasedinlacunaewithinthecalcifiedmaterialａndbecomeosteocytes.Astheskeletonreachesmaturity,thenumberａndsizeofosteoblastsdecrease;butdormantcellsremaincapableofrespondingtoinjuryａndproducingnewboneasinthehealingofafracture.Inlaterlife,theactivityofosteoblastsisinfluenceddirectlybycirculatinglevelsofparathyroidhormone.Osteoblastsexpressprotease-activatedreceptor-1ａndvescularendothelialcellgrowthfactor[1].StudiesshowthatLeukemiainhibitoryfactorcanbindtotheosteoblastcellsurfaceａndinduceboneformationbothinvitroａndinvivo[2].Thebalancebetweenosteoblastrecruitment,proliferation,differentiationａndapoptosisinsuturesbetweencranialbonesisessentialforcalvarialboneformation[3].

HCOfromScienCellResearchLaboratoriesareisolatedfromhumancalvariae.HCOarecryopreservedatprimaryculturesａnddeliveredfrozen.Eachvialcontains>5x105cellsin1mlvolume.HCOarecharacterizedbythecytochemicallydetectionofAPａndmineraldeposition.HCOarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastａndfungi.HCOareguaranteedtofurtherexpandfor15populationdoublingsintheconditionsprovidedbyScienCellResearchLaboratories.