HM

时间 : 2009-11-29 10:52:59 来源：www.chem17.com

[摘要]

恒大百盛生物（北京）科技发展有限公司上海办事处专业供应销售HM-0526肾胚胎性恶性混合瘤，欢迎您来电咨询HM-0526肾胚胎性恶性混合瘤的详细信息！恒大百盛生物（北京）科技发展有限公司上海办事处提供的HM-0526肾胚

【详细说明】

规格：1ml3ml0.1ml（浓缩）0.2ml（浓缩）阳性对照（片）试剂名：肾胚胎性恶性混合瘤试剂名（英）：Wilm’sTumorProtein（WT1）产品编号：HM-0526阳性部位：细胞核鼠抗人：单克隆抗体阳性对照：Wilm?s瘤克隆号：WLM04效价：1：25-1：50预处理：柠檬酸应用：冰冻/石蜡

Wilm＇sTumorProtein（WT1）

MouseMonoclonalAntibody

Cat.No.HM-0526

SpecificityａndComments:

Recognizesa47-55kDatumorsuppressorprotein,identifiedasWilm＇sTumor（WT）protein.EpitopeofAb-4mapsbetweenaa1-318.WT,asporadicａndfamilialchildhoodkidneytumor,isgeneticallyheterogeneous.Wilms＇tumorisassociatedwithmutationsofWT1,azinc-fingertranscriptionfactorthatisessentialforthedevelopmentofthemetanephrickidneyａndtheurogenitalsystem.TheWT1geneisnormallyexpressedinfetalkidneyａndmesothelium,ａnditsexpressionhasbeensuggestedasamarkerforWilmstumorａndmesothelioma.Thisantibodyisexcellentforstainingofformalin-fixed,paraffin-embeddedtissues.

SpeciesReactivity:Human.Othersnot-tested.

CloneDesignation:WLM04

IgIsotype/LightChain:IgG1/6.

Immunogen:RecombinanthumanWT1proteincorrespondingtoaa1-318.

Hybridoma:ProducedbyfusionbetweenBALB/csplenocytesａndmyelomaNS1cells.

SuppliedAs:200ug/mlofantibodypurifiedfromascitesfluidbyProteinGchromatography.Preparedin10mMPBS,pH7.4,with0.2%BSAａnd15mMsodiumazide.

ApplicationsａndSuggestedDilutions:

Immunohistology（Frozen&formalin/paraffin）（UseAbat1:25-1:50for60minatRT）

*[Stainingofformalin-fixedtissuesREQUIRESboilingtissuesectionsin10mMcitratebuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20min.]

Theoptimaldilutionforaspecificapplicationshouldbedeterminedbytheinvestigator.

PositiveControl:SK-NEP-1cellsorWilm＇sTumor.

CellularLocalization:Nuclear.

StorageａndStability:Storevialat4/C.

ImportantNote:Everytimebeforeyouopenthevial,werecommendbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer＇scapbecausesmallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvialduringshipmentａndstorage.

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