HM

时间 : 2009-11-29 21:04:53 来源：www.gkzhan.com

[摘要]

上海锐聪科技发展有限公司专业供应销售HM-0461视网膜母细胞瘤抑制蛋白，欢迎您来电咨询HM-0461视网膜母细胞瘤抑制蛋白的详细信息！上海锐聪科技发展有限公司提供的HM-0461视网膜母细胞瘤抑制蛋白不仅具有国内外领先

【详细说明】

规格：1ml3ml0.1ml（浓缩）0.2ml（浓缩）阳性对照（片）试剂名：视网膜母细胞瘤抑制蛋白试剂名（英）：RetinoblastomaSuppressorProtein产品编号：HM-0461阳性部位：细胞核鼠抗人：单克隆抗体阳性对照：正常结肠/乳腺癌克隆号：1F8（Rb1）效价：1：50-1：100预处理：EDTA应用：冰冻/石蜡

Retinoblastoma（Rb）SuppressorProtein

MouseMonoclonalAntibody

Cat.No.HM-0461

SpecificityａndComments:

Recognizesa105kDaphosphoprotein,identifiedasretinoblastoma（Rb）geneproduct.Itsepitopeislocalizedbetweenaa703-772.Itshowsnocrossreactionwithp107orp130.ItspecificallystainsthenucleiofBT-20cellsａndprimaryhumanforeskinfibroblast（HFF）cells.ItdoesnotstaintheRb-negativeBT549cells.This1F8/Rb1antibodyreactswiththehyperphosphorylatedaswellastheun（under）phosphorylatedformoftheRbprotein.InWesternblotting,1F8/Rb1MabdetectedRbproteinin15%ofleukemiasampleswhichwereotherwisetestednegativewithanotherantibodytoRb（Clone3C8）.

SpeciesReactivity:Human.Doesnotreactwithmouseａndrat.Others-notknown.

CloneDesignation:1F8（alsoknownasRb1）

IgIsotype:IgG1

Immunogen:Rb-$-Galactosidasefusionprotein.

Hybridoma:ProducedbyfusionbetweenBALB/csplenocytesａndmousemyelomaSp2/0Ag14cells.

SuppliedAs:200ug/mlantibodypurifiedfromtheascitesfluidbyProteinGchromatography.Preparedin10mMPBS,pH7.4,with0.2%BSAａnd15mMsodiumazide.

KnownApplicationsａndSuggestedWorkingDilutions:

Immunohistology（Frozen&formalin/paraffin）（1:50-1:100for60minuteatRT）

*（Stainingofformalin-fixedtissuesREQUIRESboilingtissuesectionsin1mMEDTA,pH8.0for10-20minfollowedbycoolingatRTfor20min.）

Theoptimaldilutionforaspecificapplicationunderagivensetofexperimentalconditionsshouldbedeterminedbytheinvestigator.

PositiveControl:ML-1,U2OS,orMolt-4cells.Normalcolon.About80%ofbreastcarcinomascontainvaryinglevelsofRbprotein.Useintermixednormalstromalnucleiasinternalpositivecontrol.

AlterationsinRBgeneresultineitherdecreasedorcompletelossofexpressionoftheRbprotein.

CellularLocalization:Nuclear.

StorageａndStability:Storevialat4/C.

ImportantNote:Everytimebeforeyouopenthevial,werecommendbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer＇scapbecausesmallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvialduringshipmentａndstorage.